Vol. 11, Special Issue 4 (2022)

Author(s):
Arunima S and Satish Verulkar

Abstract:
Protein quantification is a critical step in protein analysis, and it has several applications in clinical laboratory techniques as well as the study of biochemistry. There are numerous protein quantification methods. In this study, three protein quantification techniques (Biuret, Lowry, and Micro-Kjeldahl) were compared and found to produce consistent findings for samples with lower protein percentages. However, if the protein concentration is greater than 50%, the Lowry and Biuret procedures deviate from the Micro-Kjeldahl method. The sensitivity range of both the Biuret and Lowry techniques can be enhanced by dilution of samples prepared for spectrometric analysis without altering the standard curve. Protein estimation was performed at various degrees of dilution and reported to generate almost identical results to the Micro-Kjeldahl technique at 100 times dilution of the original sample solution prepared for analysis.