Phytochemical screening, antioxidant activities and quantification of compounds by HPLC of the leaf extracts from two varieties of Vitex negundo to explore their potential for textile uses
Author(s):
Manpreet Kaur and Dr. Geeta Mahale
Abstract:
The present study was designed to explore phytochemical bioactive compounds present in Vitex negundo leaves (Black and White). Medicinal plants are an important source of phytochemicals that offer traditional medicinal treatment of various ailments. Leaf samples of the both selected varieties growing in the ecological conditions were collected, washed, air-dried and milled. The samples were extracted with two solvent namely ethyl alcohol and distilled water. Results revealed that ethanolic extraction depicted the higher concentration of saponins, alkaloids tannins, steroids, terpenoids and flavonoids in both the varieties of Vitex negundo as compared to aqueous medium. Total phenolic content of V1 leaves extract estimated as gallic acid equivalent was found to be higher in ethnolic medium 3.17 mgGAE/g as compared to V2 i.e 2.76 mgGAE/g. Also, Total flavonoid content of leaves extract and estimated as catechin equivalent was found to be higher in ethanoic medium of V1 2.79 mgCE/g as compared to V2 1.85 mgCE/g respectively. Antioxidant activity is assessed by ABTS radical scavanging activity was also found to be higher in ethanolic extract of V1 91.64% as compared to V2 69.32%. FTIR analysis reveals the presence of various functional groups, viz. alkenes, amines, carboxylic acids, alcohols, phenols, carboxylic acids and aromatic compounds. Its major constituents myrcetin, quercetin, kaempferol and chlorogenic acid were determined by high performance liquid chromatography (HPLC) and found to be higher in ethanolic medium of V1 as compared to V2. This study suggests that the ethanolic extract from Vitex negundo leaves has strong antioxidant potential and could be a significant source of natural antioxidants and antimicrobials for functional finishing of textiles.
How to cite this article:
Manpreet Kaur and Dr. Geeta Mahale. Phytochemical screening, antioxidant activities and quantification of compounds by HPLC of the leaf extracts from two varieties of Vitex negundo to explore their potential for textile uses. The Pharma Innovation Journal. 2022; 11(3S): 1665-1672.