Toll Free Helpline (India): 1800 1234 070

Rest of World: +91-9810852116

Free Publication Certificate

Vol. 11, Special Issue 3 (2022)

Detection of anthelmintic resistance in goats of an organized farm of Assam through in vitro egg hatch assay

Author(s):
A Hafiz, DN Kalita and M Islam
Abstract:
A study was undertaken to determine resistance against albendazole (ALB), fenbendazole (FBZ) and closantel (CLS) in gastrointestinal nematodes of goat in an organized farm of Assam. The resistance was first investigated using faecal egg count reduction test (FECRT) in naturally infected goat which was further confirmed by in vitro egg hatch assay (EHA). Pre- and post-treatment copro-culture was performed to identify the species and genera of nematodes. In an organized goat farm of Assam the anthelmintics like albendazole, fenbendazole and closantel were found to be resistant i.e. the percent efficacy was not observed more than 95% of these three anthelmintics. The ED50 value for fenbendazole, albendazole and closantel was recorded as 0.3506 mg/ml, 0.3357 mg/ml and 0.2330 mg/ml, respectively. The relative potency of closantel with respect to fenbendazole was found to be 66.46 per cent indicates small amount of closantel is sufficient to 0.3506 amount of fenbendazole. In vitro egg hatch assay after logistic fit of response using fenbendazole, albendazole and closantel were estimated. The results of the survey indicated multiple resistance in Haemonchus contortus and Trichostrongylus spp. to benzimidazoles and closantel in this farm.
Pages: 792-795  |  271 Views  92 Downloads
How to cite this article:
A Hafiz, DN Kalita and M Islam. Detection of anthelmintic resistance in goats of an organized farm of Assam through in vitro egg hatch assay. The Pharma Innovation Journal. 2022; 11(3S): 792-795.

Call for book chapter