Toll Free Helpline (India): 1800 1234 070

Rest of World: +91-9810852116

Free Publication Certificate

Vol. 11, Special Issue 2 (2022)

Prevalence and molecular characterization of cattle and human rotavirus

Author(s):
Neha Singh, PR Suryawanshi, K Kusum, Raghavendra P Mishra and SS Gaikwad
Abstract:
Background: Rotaviruses are members of the Reoviridae family and one of the major etiological agents responsible for causing acute, severely dehydrating watery diarrhea. The current study was aimed to fined out prevalence rate and molecular characterization for detection of Rotavirus in cattle and human.
Method: In the present study, 31 cattle faecal and 34 human stool samples were screened for rotavirus by RNA-PAGE and RNA PAGE positive samples were confirmed positive using RT-PCR.
Results: Four cattle samples and 11 human stool samples were found positive by RNA-PAGE. It was observed that 4 cattle calf faecal samples were found positive with a typical 4:2:3:2 electropherotype migration pattern of group A rota virus. In case of human stool samples 11(28.9%) were found positive for rotavirus by RNA-PAGE that too belonged to group A rotavirus. Out of the 15 PAGE positive samples, 2 cattle and 3 human samples were confirmed positive for Rotavirus by RT-PCR.
Pages: 1589-1592  |  213 Views  43 Downloads
How to cite this article:
Neha Singh, PR Suryawanshi, K Kusum, Raghavendra P Mishra and SS Gaikwad. Prevalence and molecular characterization of cattle and human rotavirus. The Pharma Innovation Journal. 2022; 11(2S): 1589-1592.

Call for book chapter