Vol. 11, Special Issue 2 (2022)

Author(s):
Aeknath Virendra, Sarita U Gulavane, Ravindra J Chaudhari, Sandeep M Gaikwad, Raju R Shelar, Shailesh D Ingole, Varsha D Thorat, Zulfikar A Ahmed and Ravi Reddy

Abstract:
Endometritis, which affects around 60% of barren mares, is one of the most common causes of subfertility and infertility in breeding mares. The diagnosis of endometritis can be done by various methods. Heat shock protein (HSP-90) are significantly produced by heat shock and other kinds of chemical and physical stress. In some studies, shot gun proteomics was also used to identify heat shock proteins in mares suffering from endometritis. Annexins 1 to 5 are expressed locally in the horse's endometrium, with ANXA1 and ANXA2 being the most prevalent. The present study was designed to analyse the presence of HSP90, Annexin A1 and A2 biomarkers in low volume uterine lavage as well as serum samples from healthy as well as mares suffering from endometritis. The results indicated that above mentioned biomarkers were found in the low volume uterine lavage samples as well as in the serum samples but the concentration was found to be very low, and no significant difference was found between the values of optical density obtained by the ELISA reader. The reason for the results obtained could be the actual low concentration of these biomarkers present in the uterine lavage samples as well as in serum samples of the mares. It can be concluded that the analysis of HSP-90, Annexin A1 and A2 biomarkers by using ELISA is not remarkable for the diagnosis of endometritis in mares.