Protein hydrolysate extraction from chicken intestine by enzymatic hydrolysis
E Vimalraj, R Ramani, V Appa Rao, M Parthiban and R Narendrababu
Protein hydrolysate extracted from chicken intestine is rich in proteins and peptides having a potential for using as a functional ingredient in animal feeds. Protein hydrolysate was found to have better nutritional and functional properties that could be used as feed additives, which are bioactive and may exert beneficial physiological effects. In this study, protein hydrolysate was extracted from chicken intestine by enzymatic hydrolysis using protease P fungal enzyme. Parameters like yield, physical, chemical properties; proximate analysis, degree of hydrolysis and nitrogen recovery were studied in the extracted protein hydrolysate. The yield of protein hydrolysate was 21.37 ±0.33, pH was 6.6 ± 0.02 and instrumental colour values of Lightness (L*) 37± 0.87, Redness (a*) 0.87 ± 0.03, yellowness (b*) 12.22 ± 0.43, Hue 85.41± 0.42 and Chroma value 12.26 ± 0.42. The protein hydrolysate was having proximate composition of 3.4 ± 0.1 per cent of moisture, 63.63 ± 1.09 per cent of protein, 4.38 ± 0.16 per cent of fat, 19.38 ± 0.57 per cent of carbohydrates and 9.2 ± 0.54 per cent of ash. The degree of hydrolysis of protein hydrolysatewas36.08 ± 0.73 per cent respectively. The nitrogen recovery of the extracted protein hydrolysate was 68.39 ± 1.24 per cent. Thus, the waste from poultry industry i.e. chicken intestine can be effectively utilized by transforming in to protein hydrolysate, which in turn finds possible applications in aqua feed, as a protein supplement and as feed additives for weanlings and can also be further used in the extraction of low molecular bioactive peptides.
How to cite this article:
E Vimalraj, R Ramani, V Appa Rao, M Parthiban and R Narendrababu. Protein hydrolysate extraction from chicken intestine by enzymatic hydrolysis. The Pharma Innovation Journal. 2022; 11(2S): 57-62.