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Vol. 11, Special Issue 1 (2022)

Molecular detection of Babesia bigemina in the natural cattle tick vector Rhipicephalus annulatus of Kerala by the nested PCR

Author(s):
Prabodh Kumar Hembram, Gatchanda Shravan Kumar, Anooja M, Kavya S, Ihsan Mohammed Naser, Amal Sebastian, Anju Varghese, Deepa CK, Ajith Kumar KG, Dinesh CN and Reghu Ravindran
Abstract:
The objective the present study was to detect the Babesia bigemina DNA in the Rhipicephalus annulatus ticks engorged on domestic cattle of Northern part of Kerala, South India. A total of 15 engorged R. annulatus female ticks (n=15) were collected. The DNA isolated from the ticks was used as template for the nested PCR amplification of rhoptry-associated protein-1a (rap-1a) gene specific for B. bigemina. Out of 15 ticks examined, the pathogen DNA was detected in seven ticks.
Pages: 996-998  |  286 Views  67 Downloads
How to cite this article:
Prabodh Kumar Hembram, Gatchanda Shravan Kumar, Anooja M, Kavya S, Ihsan Mohammed Naser, Amal Sebastian, Anju Varghese, Deepa CK, Ajith Kumar KG, Dinesh CN and Reghu Ravindran. Molecular detection of Babesia bigemina in the natural cattle tick vector Rhipicephalus annulatus of Kerala by the nested PCR. The Pharma Innovation Journal. 2022; 11(1S): 996-998.

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