Vol. 11, Special Issue 1 (2022)

Author(s):
Prabodh Kumar Hembram, Gatchanda Shravan Kumar, Anooja M, Kavya S, Ihsan Mohammed Naser, Amal Sebastian, Anju Varghese, Deepa CK, Ajith Kumar KG, Dinesh CN and Reghu Ravindran

Abstract:
The objective the present study was to detect the Babesia bigemina DNA in the Rhipicephalus annulatus ticks engorged on domestic cattle of Northern part of Kerala, South India. A total of 15 engorged R. annulatus female ticks (n=15) were collected. The DNA isolated from the ticks was used as template for the nested PCR amplification of rhoptry-associated protein-1a (rap-1a) gene specific for B. bigemina. Out of 15 ticks examined, the pathogen DNA was detected in seven ticks.