Vol. 11, Special Issue 1 (2022)

Author(s):
BK Prasad, G Singh, R Chandra and AK Sharma

Abstract:
Gene transfer through genetic engineering requires an efficient protocol through which complete plants can be regenerated. Thus, this study was conducted to develop a suitable protocol for plantlet regeneration from different explants (viz. cotyledon and plumule explants derived from 5 day old seedlings) of five different cultivars of Indian mustard. These explants were cultured on MS medium supplemented with 1.0 mg/l BAP and 0.2 mg/l IAA. Varuna exhibited significantly higher frequency (85%) of shoot regeneration than other genotypes, whereas RH 30 exhibited more number of shoot regeneration per explant but the frequency of shoot regeneration in this variety was poor than that of Varuna. The explants, cotyledon and plumule from Varuna produced the highest frequency (75% and 95%) of shoot regeneration, whereas both the explants from RH 30 produced the highest number of shoots per explant. It is clear that different genotypes and explants have different potential for frequency of shoot regeneration as well as number of shoot regeneration per explant.