Vol. 11, Special Issue 11 (2022)

Author(s):
Mayur Patil, Pramod Borkar, Paresh Potphode, Shrikant Rite, Hemant Pawar and Mahesh Kulkarni

Abstract:
Six bacterial isolates were obtained from rhizosphere of brinjal, papaya, tomato, okra and Chilli from different pockets of Konkan region. The results of cultural and biochemical tests -Gram staining, KOH test, Catalase test, Starch Hydrolysis, Gas formation test and Methyl red test revealed that all the six isolates were negative to Gram stain and Methyl red test and positive to other tests. In case of H₂S production test the two isolates viz., Pf4 and Pf6 showed positive results while others showed negative results. All the isolates exhibited yellow colony pigmentation and emitted bluish green fluorescens on exposure to UV radiation. On the basis of these results, the isolates were identified as strains of P. fluorescens. The bio-control ability of all the strains was evaluated against three fungal – Sclrerotia, Rhizoctonia, Fusarium and one bacterial plant pathogen -Ralstonia solanacearum. The results revealed that the Pf1 strain isolated from brinjal rhizosphere was the most promising against Fusarium, Rhizoctonia and Ralstonia while Pf4 isolated from okra field was effective against Sclerotium.