Vol. 11, Special Issue 11 (2022)

Author(s):
Pradipkumar Parmar, Ashish Patel, Mayank Patel, Prakash Koringa and RS Joshi

Abstract:
The present study was undertaken for evaluation of genetic diversity of indigenous chicken Mewari of southern and central Rajasthan by using 24 microsatellite markers in eight multiplexed set. A total of 245 alleles were found in present study where the observed number of alleles per locus varied from 3 (ADL 210) to 21 (ADL 136). The mean observed numbers of alleles were found to be 10.21. The overall means for observed and expected heterozygosity were 0.560 and 0.810 respectively, with the ranges of 0.182 (ADL 39) to 0.927 (MCW 59) and 0.601 (ADL 210) to 0.926 (ADL 136), respectively. The Fixation index (FIS) value for all 24 microsatellite loci ranged from -0.088 (ADL 210) to 0.757 (ADL 39) with mean of 0.280. Polymorphic Information Content (PIC) value was ranging from 0.503 (HUJ 2) to 0.949 (ADL 136) with a mean of 0.813. The test for genetic equilibrium indicated that out of total 24 microsatellite loci, 22 microsatellite loci were found deviated significantly from Hardy-Weinberg Equilibrium. The high number of observed alleles and high heterozygosity indicated presence of high genetic variability in Mewari chicken which is indigenous native of southern and central Rajasthan.