Abstract:A clinically infected female Holstein Friesian cross-bred, age 5 years with the symptoms of anorexia, dullness and decline of milk yield, pale mucous membrane, swelling pre-scapular superficial lymph node and temperature 106 °F was diagnosed for acute theileriosis by microscopy and PCR. Microscopic examination recorded 85% of parasitaemia. The piroplasms in parasitized RBCs per field were counted and recorded 6, 5, 4, 3, 2, 1 piroplasms in 1, 2, 6, 10, 45, and 80 RBCs, respectively. Haematological values viz. haemoglobin (Hb gm/dl), packed cell volume (%) and total erythrocyte count (x106/ µl) were drastically reduced to 6.2 (gm/dl), 18% and 1.92 x 106/µl, respectively. Animal was treated with single dose of Buparvaquone (Butalex) @2.5 mg/kg.bwt i/m and hematinic (Ferritas) 10 ml i/m, and Dexamethasone 4ml s/c, Meloxicam 10 ml i/m for 3 days along with 5% dextrose as supportive therapy. Improvement on the health of animals was recorded after 3rd day post treatment.