Vol. 11, Special Issue 11 (2022)

Nanotechnology is one of the emerging area of research interest in the present era. Nanomedicine has been developed for a number of diseases and has been tested in the different disease models. However, evaluating the safety of nanomedicine is an important aspect. In the present study, bilirubin nanoparticles were synthesized by our novel method and characterized for different parameters such as hydrodynamic diameter, zeta potential, UV-Visible spectroscopy analysis, and transmission electron microscopy. The bilirubin nanoparticles had hydrodynamic diameter of 100-150 nm, negative surface charge and spherical morphology. The synthesized nanoparticles were quite stable as observed in UV-visible spectroscopy. This nanoformulation was tested at three different concentrations i.e. 0.03%, 0.1% and 0.3% for safety evaluation upon topical application in wounded rats in which it was applied twice daily for 21 days. Evaluation for safety profile was carried out in terms of haematological and biochemical parameters and necropsy findings at 21 days post-wounding. The haematology and biochemical parameters showed no significant difference in comparison to control group. In case of necropsy, the vital organs such as liver, kidney, heart, spleen, lungs etc., showed no pathological evidence. Therefore, the synthesized nanoparticles had no systemic effects upon topical application.