Vol. 11, Special Issue 11 (2022)

Author(s):
Rathod PG, Singh VK, Choravada DR, Padodara RJ, Odedara AB, Solanki GB and Javia BB

Abstract:
Leukocytospermia is a condition in which a high count of white blood cells (WBCs) is present in semen. In this study, the cattle spermatozoa were in-vitro cultured in presence of cattle white blood cells to evaluate the effect of WBCs on physico-morphological (motility, viability, membrane integrity and acrosomal integrity) parameters and lipid peroxidation of bovine spermatozoa. A total of eighteen ejaculates were used to co-culture one million spermatozoa with, 0, 50, 500 or 5000 white blood cells (WBCs) per millilitre, respectively in Sperm-Tyrode's Albumin Lactate Pyruvate (Sp-TALP) media for 4 hours at 37 °C. The results showed that the presence of 5000 leukocytes/ million spermatozoa significantly (p<0.05) decrease physico-morphological parameters like viability, motility, membrane integrity and acrosome integrity and significantly (p<0.05) increase lipid peroxidation of Gir bull spermatozoa when cultured in-vitro for 4 hours. Hence, we conclude that 5000 or more leukocytes per million spermatozoa adversely affect physico-morphology and lipid peroxidation of bovine spermatozoa resulting in reduction in functional competence of bull semen.