Vol. 11, Special Issue 11 (2022)

Background: Food poisoning health risk due to the consumption of contaminated food and antibiotic-resistant bacteria is currently a serious threat of public health.

Aims: This study aimed to determine the occurrence and antibiogram of Staphylococcus aureus isolated from bovine raw and bulk milk samples from pari-urban of Navsari and Surat, Gujarat.

Methods: A total of 120 samples comprising of fresh raw milk, cattle and buffalo pooled and mix bulk milk were collected standard microbiological procedures. Moreover, we evaluated 17 different antimicrobial agents on S. aureus for susceptibility and resistant by using D-test.

Results: Out of the 120 milk samples examined, 40 S. aureus were isolated showed a prevalence of 33.33% and all isolates were successfully amplified 16S rRNA gene-based PCR. Antimicrobial sensitivity test of conformed all 40 isolates indicated phenotypes were completely to moderately resistance to Penicillin G followed by Cefixime, Cephotaxime, Methicillin and Oxytetracycline and other antibiotics were observed significance high susceptibility to Tylosin followed by Ampicillin, Gentamicin, Tetracycline, Chloramphenicol, Ciprofloxacin. All isolates showed a MAR index ranging between 0.06 to 0.47, after performing the correction for pairs of variables with a statistically significant Pearson correlation coefficient showed highest correlation of compounds from same class to other class and high correlation were yielded for aminoglycosides, quinolones and beta-lactams.

Conclusion: This study indicated the potential misuse of antibiotic and public health risks due to staphylococcal food poisoning were conditions congenial supports the bacterial growth.