Vol. 11, Special Issue 10 (2022)

Author(s):
Mayank Pratap, AK Singh, RP Vyas, Sanjay Kumar Tripathi, Rajesh Kumar Pal and Shubham Kumar Srivastava

Abstract:
Brahmi is medicinal herb growing in muddy and subtropical latitudes with very good economic potential. It has been used as botanical medicines in many countries for thousands of years, they are used for preparing food, feed, and beverages, formulating ethno medicinal and ethno veterinary remedies and preparing traditional and modern cosmetic products. An efficient protocol for rapid in vitro propagation of valuable medicinal plant brahmi by using shoot tip and nodal cutting as explants were done. Brahmi is likely to become a major source of a number of medicinal products of high value in the coming future. This research work was carried out in Tissue Culture Laboratory, College of Horticulture of Chandra Shekhar Azad University of Agriculture and Technology, at Kanpur. Different experiments were conducted in order to achieve the objectives of the present investigation. The present work deals with the establishment of a viable protocol for this valued medicinal plant. Out of ten different treatments regime tried for induction and multiplication on shoots explants^-1. Taken two types of media i.e. MS media and B₅ media supplemented with different doses of plant growth regulators i.e. 1 mg/L of BAP, 2 mg/L BAP, 3 mg/L BAP, 4 mg/L BAP and 5 mg/L BAP for shoot induction and data to be recorded at weekly interval from first week to six weeks. Resulted that media MS was given best response at weekly interval, supplemented with different doses of plant growth regulators (i.e. 1 mg/L of BAP, 2 mg/L BAP, 3 mg/L BAP, 4 mg/L BAP and 5 mg/L BAP) for shoot induction per explants of brahmi as compare to B₅ media. Doses 3.0 mg/L BAP supplemented in MS media and B5 media was found best to induce maximum number of shoots per explants followed by 4.0 mg/L BAP in brahmi. Explants nodal cutting was done highly shoot proliferation with MS media and doses of plant growth regulators as compare to shoot tip explants of Brahmi.