Vol. 11, Special Issue 10 (2022)

Author(s):
Md. Zakiuddin, MK Tandle, SM Usturge, NA Patil, D Dilip Kumar, VR Kasaralikar and MD Suranagi

Abstract:
The study was conducted to determine the effect of GnRH (Buserelin Acetate) administration as a therapy for repeat breeding syndrome in 30 buffaloes. White side test was carried out to exclude the possibility of sub-clinical genital infection in these animals. The repeat breeder buffaloes which were found to be negative for White side test were divided randomly into three equal groups (n=10). The Group I buffaloes received GnRH analogue 10μg at the time of insemination, Group II received GnRH analogue 10μg at the time of insemination and another dose of GnRH analogue 10μg on 12^th day post insemination. Group III kept as control. The Vaginal Electrical Resistance (VER) values of pregnant (242±6.27) and non-pregnant buffaloes (262.04±7.07) differ significantly (p<0.05). The color of estrual mucus in repeat breeder buffaloes was 62.50% transparent, 37.50% translucent and 00.00% whitish. 16.67%, 45.83% and 37.50% of repeat breeder buffaloes were having thick, viscous and thin consistency of estrual mucus. The mean pH value of estrual mucus was 7.37±0.04. Conception rate in buffaloes is more when cervico-vaginal mucus on the day of estrus is transparent, viscous, with typical fern pattern, spinnbarkeit value more than 10 cm and pH ranges 7-8. Conception rate in repeat breeder buffaloes was 90%, 70% and 10% in Group II, I and III respectively.