Vol. 11, Special Issue 10 (2022)

Author(s):
CH Priyadarshini, P Eswara Prasad, K Aswani Kumar, D Nagalakshmi and D Madhuri

Abstract:
Saraca asoca (Roxb.) is used as traditional herbal medicine. The plant contains phytoconstituents like flavonoids, steroids, glycosides, saponins, tannins. Among the phytosterols, β-sitosterol is major compound which has pharmacological activities such as antimicrobial, anticancer, immunomodulatory, anti inflammatory, hepatoprotective, antioxidant and also acts on various gynecological disorders. Due to its pharmacological importance, the main aim of present study is to determine the quantity of β-sitosterol in Saraca asoca leaves in methanol and aqueous extracts by HPLC technique. In this method, an isocratic binary system of acetonitrile and ethanol (70:30 v/v), a flow rate of 1ml/min with 198 nm UV detection and was maintained at 30 °C temperature. The retention time obtained for standard concentration of different dilutions of 0.05, 0.5 and 5 (mg/ml) were 25.793, 25.833 and 25.833 min respectively and for methanol and aqueous extracts of Saraca asoca leaves showed 25.860 and 25.793min respectively. The calibration curve constructed based on three dilutions of β-sitosterol standard at concentrations produced a linear regression (r = 0.999) and the concentration ofwere 3.37 X 10^-4 mg/ml and 4.33 X 10^-5mg/ml respectively.