Vol. 10, Special Issue 8s (2021)

Author(s):
Arun R, K Krishnakumar, S Balasubramanian and Dr. H Gopi

Abstract:
Twenty healthy, parous, cyclic after day 60 post partum crossbred cows with no palpable abnormalities in the reproductive tract were selected for this study. The selected cows were randomly divided into Group I and II. Group I served as control and Group II received CIDR insert between 9 and 12 of the oestrous cycle and considered as day 0. The PGF2α was administered on day 8 after CIDR insert. Group I cows were inseminated twice at 24 h interval during natural oestrus and Group II cows were inseminated twice at 48 and 72 h with good quality frozen thawed semen after CIDR insert removal. Pattern of natural and synchronized oestrus were studied and follicle and CL diameter were measured during different phases of oestrous cycle by trans-rectal ultrasonography in both groups. Blood sample were collected during normal oestrus, day 11 and 12 of CIDR insert and 10th day of post insemination for progesterone assay. Pregnancy was confirmed on day 45 post insemination by rectal palpation and ultrasound examination. The accumulated data were analyzed statistically.