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Vol. 10, Special Issue 8 (2021)

Oxidative stress induced hematological alteration of methotrexate attenuates Bacopa monnieri in rats

Author(s):
Tummala Srinivas, Dr. B Anil Kumar, BDP Kala Kumar, M Lakshman and A Gopala Reddy
Abstract:
The ameliorative potential of aqueous leaf extract of Bacopa monnieri and silymarin were studied against methotrexate induced heamatological alteration. Total 36 male Wistar rats were separated into six groups (n = 6). Group 1 was maintained as normal control. A single dose of Methotrexate (20 mg/kg i.p.) was administered on day 9 to groups 2, 4, 5 and 6. Groups 3 and 5 were administered (p/o) with aqueous leaf extract of Bacopa monnieri @ 300 mg/kg bodyweight. Group 4 was administered (p/o) with aqueous leaf extract of Bacopa monnieri @ 150 mg/kg bodyweight and while group 6 received silymarin @ 200mg/kg body weight. The treatment was given for a period of 14 days. The animals were sacrificed on day 15th of the experimental. The blood was drawn for hematology and serum was collected for assessing total protein, biochemical parameters, and liver tissue was collected for estimation of antioxidant markers and tissue inflammatory markers. This current study revealed significant alterations in Hematology (RBC, WBC, Hb and PCV), antioxidant profile (TBARS, GSH and SOD), in methotrexate control group 2 as compared to treatment groups 4 and 5. There was significant improvement in group 5 and the values were comparable to treatment group 3. The values of groups 3 and 1 were comparable without any significant difference. Groups 4, 5 and 6 showed significant improvement in all the parameters compared to group 2.
In conclusion, Bacopa monnieri was found to possess the ameliorating action against methotrexate induced heamatological changes as similar to silymarin and results were more pronounced in the group receiving the higher dose, which was evident in this study by reducing the toxic markers and restoration of antioxidant enzymes. The overall beneficial effects of Bacopa monnieri could be attributed to its antioxidant actions.
Pages: 72-76  |  647 Views  193 Downloads
How to cite this article:
Tummala Srinivas, Dr. B Anil Kumar, BDP Kala Kumar, M Lakshman and A Gopala Reddy. Oxidative stress induced hematological alteration of methotrexate attenuates Bacopa monnieri in rats. The Pharma Innovation Journal. 2021; 10(8S): 72-76.

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