Vol. 10, Special Issue 7 (2021)

Author(s):
Ganapathi P, Murali N, Saravanan R, Raja A and Selvaraju M

Abstract:
Among the many genes governing sperm functions, a group of genes have been reported to control ion channels that play an important role in sperm motility. They were named the cation channel of sperm (CatSper) and further known to have five members, viz. CatSper 1, 2, 3, 4, and β (7). Any mutations in them were proposed as the cause of poor motility in sperms and infertility. Nucleotide alterations pertaining to Catsper gene cause poor motility in sperm production and infertility. Among the calcium-permeable channels present on sperm cells, only CatSper1 and 2 are known to be indispensable for male fertility. Exon 2 of CatSper2 gene was amplified and sequenced. There are two nucleotide changes observed, conversion of C nucleotide in 276^th position to A nucleotide (g.276C>A) (Transversion) and conversion of A nucleotide to C in the 277^th position (g.277A>C). In the translation process, the nucleotide changes at 276^th and 277^th position (g.276C>A and g.277A>C) does not reveal any amino acid change in the amino acid sequence, in both Jersey crossbred bulls and Holstein Friesian crossbred bull.