Vol. 10, Special Issue 7 (2021)

Author(s):
Sakthivel Duraisamy, Ramesh Srinivasan, Karthick Venkatesh and Sriram Padmanabhan

Abstract:
The development and validation of a simple accurate method based on HPLC with ultra-violet detection for the quantification of tilmicosin in chicken plasma and meat is described. Tilmicosin is a popular macrolide antibacterial used in poultry for the control and therapy of Mycoplasmosis in poultry. There is a need to assay measure Tilmicosin concentrations in plasma and meat of chicken for pharmacokinetics and residue studies. This paper attempts to describe a simple sensitive HPLC assay. Tilmicosin was extracted from the plasma using a single-step deproteinization by perchloric acid. Chromatographic separation of tilmicosin from interfering components was achieved with a C18 column, mobile phase consisting of 0.1m ammonium formate + acetonitrile + methanol in the ratio of 60:30:10 v/v (pH adjusted with trifluoroacetic acid) was used. The chromatogram was monitored at a wavelength of 287 nm. The column temperature was maintained at 30 °C. The tilmicosin standard peaks in the plasma observed at the retention time of 7-7.5 min. The method was linear from 0.05 to 5µg.ml^-1 (r²≥0.998), and tilmicosin had a mean recovery from plasma of 100.66%. The coefficient of variation of inter-day and intra-day quality control samples was less than 15%. The method described is suitable for chicken meat and plasma and is easily adoptable in analytical laboratories.