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Vol. 10, Special Issue 7 (2021)

Validation of simple isocratic HPLC assay method for determination of tilmicosin concentration in chicken plasma and meat

Author(s):
Sakthivel Duraisamy, Ramesh Srinivasan, Karthick Venkatesh and Sriram Padmanabhan
Abstract:
The development and validation of a simple accurate method based on HPLC with ultra-violet detection for the quantification of tilmicosin in chicken plasma and meat is described. Tilmicosin is a popular macrolide antibacterial used in poultry for the control and therapy of Mycoplasmosis in poultry. There is a need to assay measure Tilmicosin concentrations in plasma and meat of chicken for pharmacokinetics and residue studies. This paper attempts to describe a simple sensitive HPLC assay. Tilmicosin was extracted from the plasma using a single-step deproteinization by perchloric acid. Chromatographic separation of tilmicosin from interfering components was achieved with a C18 column, mobile phase consisting of 0.1m ammonium formate + acetonitrile + methanol in the ratio of 60:30:10 v/v (pH adjusted with trifluoroacetic acid) was used. The chromatogram was monitored at a wavelength of 287 nm. The column temperature was maintained at 30 °C. The tilmicosin standard peaks in the plasma observed at the retention time of 7-7.5 min. The method was linear from 0.05 to 5µg.ml-1 (r2≥0.998), and tilmicosin had a mean recovery from plasma of 100.66%. The coefficient of variation of inter-day and intra-day quality control samples was less than 15%. The method described is suitable for chicken meat and plasma and is easily adoptable in analytical laboratories.
Pages: 548-553  |  839 Views  555 Downloads
How to cite this article:
Sakthivel Duraisamy, Ramesh Srinivasan, Karthick Venkatesh and Sriram Padmanabhan. Validation of simple isocratic HPLC assay method for determination of tilmicosin concentration in chicken plasma and meat. The Pharma Innovation Journal. 2021; 10(7S): 548-553.

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