Vol. 10, Special Issue 7 (2021)
Next generation sequencing technologies and its applications in aquaculture
Lakshman Sahoo and Susanta Kumar Dash
Sequencing of the DNA of an organism leads to better understanding of genes and their physiological role. With the advent of NGS technologies it is now feasible to sequence whole genome sequencing and re- sequencing at faster rate in turn can be subsequently used for comparing aquatic genomes for studying functional genome diversity employing various bioinformatics tools. Massive parallel sequencing methods made it practically possible whole genome sequencing and re-sequencing of large number of aquatic genomes at much higher speed and relatively lower cost compared to traditional sequencing methods. The major gap in the genomic approaches to aquaculture improvement is in the application of genomic information for development of improved aquatic strains through next-generation sequencing technologies. The most effective effort to fulfill the gap is to integrate various research disciplines which form core components of molecular aquaculture breeding. The integration of various approaches required knowledge of whole genome organization, strong statistical knowledge to estimate the gene/genetic effects, good experience in molecular biology techniques and traditional breeding methodologies.
How to cite this article:
Lakshman Sahoo and Susanta Kumar Dash. Next generation sequencing technologies and its applications in aquaculture. The Pharma Innovation Journal. 2021; 10(7S): 348-351.