Vol. 10, Special Issue 7 (2021)

Author(s):
Kalaiselvi Lakshmanan, Gokulakannan Ragava and Ramesh Srinivasan

Abstract:
A simple QuEChERS-based (Quick, Easy, Cheap, Effective, Rugged, and Safe) analytical method for the determination of chlorpyrifos residues in chicken meat samples by gas chromatography coupled with electron capture detector was validated. QuEChERS method involves sample extraction with acetonitrile, salting out phase separation followed by clean up with dispersive solid phase extraction using primary secondary amines. The method was validated by specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision and matrix effect. The method showed good linearity with the limit of detection and the limit of quantification of 0.02 and 0.05 µg/g, respectively. The per cent recovery of this method ranged from 86.52 – 94.25% with optimal intra-day and inter-day precision (Coefficient of variation < 8.0%). The matrix effect was negative and between -20% to + 20%, complying with the standard guidelines. The validated was applied for determination of chlorpyrifos residues in 50 chicken meat samples collected from Tamil Nadu and no chlorpyrifos residues were detected in the sample analyzed. The validated method was simple, sensitive and can be routinely employed for monitoring chlorpyrifos residues in chicken meat samples.