Vol. 10, Special Issue 7 (2021)

Author(s):
Deepika Jain, Rajani Joshi, Deepika Dhuria and Manohar Sain

Abstract:
This study investigated the efficacy of autoclave and microwave for treatment of biomedical waste. As an alternative to incineration technology, a steam autoclave and microwave was used to sterilize the biomedical waste. Biological indicator strips containing Geobacillus Stearothermophilus and Bacillus atrophaeus spores were used to test the efficacy of autoclave and microwave for sterilization of biomedical waste, respectively. It was reported that steam autoclave effectively treated the biomedical waste samples at 121 °C for 30 min and 121 °C for 45 min but at 121 °C for 15 min it showed ineffective sterilization. Microwave completely destroyed the all forms of microorganisms and provides effective sterilization at temperature of 70 °C to 100 °C, frequency of 2450 MHz and cycle of 30 min to 1 hour.