Vol. 10, Special Issue 7 (2021)

Author(s):
MK Goswami, S Sinha, BC Deka, M Bhuyan and RK Biswas

Abstract:
Semen from Sirohi and Beetal bucks (three each) maintained at Goat Research Station, Assam Agricultural University, Burnihat were collected. Study on the effect of adding 2mM vitamin E and 2mM Caffeine in tris extender before and after freezing on the quality of frozen semen were conducted. Sperm motility and live sperm were studied using conventional method. Live intact acrosome was found out using Eosin-Nigrosin-Giemsa stain. HOST- reacted spermatozoa were recorded using hypo-osmotic solution having 150 mOsm/L. The overall mean percent sperm motility, live sperm, live intact acrosome, HOST-reacted sperm were significantly (P<0.05) higher after equilibration containing Vitamin E than in Caffeine as well as between treatments after freezing containing Vitamin E than in that containing Caffeine. The activities of ALT (Alanine transaminase) and AST (Asperate transaminase) (u/L) in extracellular medium were significantly (P<0.05) lower after freezing in tris extender containing vitamin E than in that containing Caffeine.