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Vol. 10, Special Issue 6 (2021)

Methods of preparation of dog erythrocytic membrane antigen

Author(s):
Kalaiselvi G, Tirumurugaan KG, G Dhinakar Raj, Vijayarani K and Baranidharan R
Abstract:
Erythrocyte membrane consists of lipid bilayer, cytoskeleton domains and the lipid domain. The structural protein tubulin was not involved in cell motility or phagocytosis and the lipid domain contain nearly equal parts of lipid and protein. Preparation and characterization of dog erythrocytic membrane are important for identification of canine blood groups. Physical method of preparation of ghost membrane showed less purity and time consuming. The chemical hypotonic osmolysis method showed high yield of membrane protein as well as less contamination with cytoplamic protein. The combination of chemical osmolysis, detergent solubilisation and sonication gave good ghost erythrocytic membrane profile in SDS - PAGE. The normal erythrocytic membrane protein pattern depends on the method of preparation and the degree of washing. Thoroughly washed membranes lack of visible band 8 and whereas globin and partially washed membranes have relatively higher quantities of bands. Small amounts of spectrin may also be lost during in vitro lysis and washing procedure.
Pages: 141-145  |  371 Views  71 Downloads
How to cite this article:
Kalaiselvi G, Tirumurugaan KG, G Dhinakar Raj, Vijayarani K and Baranidharan R. Methods of preparation of dog erythrocytic membrane antigen. The Pharma Innovation Journal. 2021; 10(6S): 141-145.

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