Vol. 10, Special Issue 6 (2021)

Author(s):
KG Chaitanya, T Madhava Rao, A Jagadeesh Babu and B Sreedevi

Abstract:
Biofilms are the most common mode of bacterial growth in nature and are also important in clinical infections. The present study was conducted to find the prevalence of biofilm formation of E. coli isolated from pig fecal samples (250), pork (120) and water samples (60) used during pig farming and during slaughter, 165 E. coli isolates were obtained from 430 samples of which 132 isolates from pig fecal samples, 17 from pork and 16 isolates from water samples. All the 165 E. coli isolates were subjected to assessment of biofilm production by modified congo red agar plate (MCRA) /Slime production method and microtitre plate method. From the 165 E. coli isolates, 95 (57.57%) and 102 (61.81%) were biofilm producers in MCRA method and microtitre plate method respectively. In the present study, biofilm production in Microtiter plate method was slightly higher than slime production. Most of the isolates (96.07%) were low biofilm producers, whereas 3.92 percent of isolates were moderate biofilm producers.