Vol. 10, Special Issue 5 (2021)
Effect of different maturation media on in vitro maturation of buffalo oocytes (Bubalus bubalis)
B Srilatha, K Ramchandra Reddy, Sathya Velmurugan, N Nalini Kumari and T Raghunandan
Buffalo is an integral part of livestock agriculture in Asia since many centuries, because they provide draught power, milk, meat and hide to millions of people, particularly small-scale farmers. The low production efficiency can be attributed to delayed puberty, higher age at calving, long postpartum anestrus period, lack of overt sign of heat and low conception rate. In the present study, three different maturation media were used for in vitro maturation. A total of 450 culture grade oocytes (replicates: 6) and three different maturation media i.e. IVM-I (TCM-199 + hormones + 10% EBS + Gentamycin), IVM-II [(TCM-199 + hormones + gentamycin) and IVM-III [(TCM-199 + Gentamycin) respectively. Results indicated that among three treatments, highest proportion (66.64±.57) of oocytes extruded PB in medium with TCM 199H + 10% EBS + hormones followed by 52.04±0.72 in medium with TCM 199H + hormones and the lowest proportion was observed in medium with TCM 199H + Gentamicin (7.39±0.23). The proportion of MII chromosomes in IVM-I was significantly (P≤0.05) higher than IVM-II (52.83±0.69) and IVM-III (8.70±1.00).
How to cite this article:
B Srilatha, K Ramchandra Reddy, Sathya Velmurugan, N Nalini Kumari and T Raghunandan. Effect of different maturation media on in vitro maturation of buffalo oocytes (Bubalus bubalis). The Pharma Innovation Journal. 2021; 10(5S): 147-149.