Vol. 10, Special Issue 4 (2021)

Author(s):
Rahul Singh, Dr. Swapna P Antony and Dr. Roshma Phlip

Abstract:
A Cyprinids species Catla catla, being investigated as future aquaculture species in countries around the world. However they face a number of issues and limitations which will need to be overcome to ensure future stability and growth, one of which is disease outbreak. In the present study, gene based approach was employed for identifying AMP genesin freshwater fishes belonging to Family Cyprinidae. Catla-catla, were taken for identification and characterization of antimicrobial peptide genes present in these organisms. A novel Histone H2A derived AMP genes could be identified and characterized from C. catla from the current study. The AMP genes were the first report of these genes from this fish species. The AMP genes were named HIS-C1. An mRNA fragment of 124 bp encoding 41 amino acid corresponding to histone H2A gene could be amplified from C. catla (HIS-C1). Multiple alignment of these genes showed the presence of conserved regions in both nucleotide and amino acid sequences. Phylogenetic tree was constructed using histone H2A sequences and this also showed the basal placement of this AMP along with other histone identified from other fishes belonging to family Cyprinidae.