Vol. 10, Special Issue 4 (2021)

Author(s):
Bosco Jose, Manoj Kumar Tripathi, Abhishek Kumar, Pranay Kumar Konda and Mihir Sarkar

Abstract:
The slaughterhouse Caprine ovaries were the cheap source of Caprine germplasm, and it was collected in a hygienic method within a short period. The slicing was the method of oocyte isolation followed in this study. The grade A and B quality oocytes were isolated, washed, and matured in a prepared media. The does in standing heat was identified and fresh buck semen was collected from trained buck available in the animal shed. The collected semen was evaluated for physical attributes, mass motility, live percentage of spermatozoa, individual motility, acrosomal integrity, and the Hypoosmotic swelling test (HOST). The semen from proven buck was used for the successful in-vitro fertilization (IVF) of oocyte and production of Caprine embryos. Various factors were affected the successful production of cleaved embryos in Caprine and were well explained by various authors. The stepwise precautions needed to be taken to successful in vitro embryo production were the main objective of this study.