Vol. 10, Special Issue 3 (2021)

Author(s):
TR Resmi, R Ambily, M Mini, N Thomas and SR Nair

Abstract:
An acute and economically significant disease, peste des petits ruminants (PPR) is caused by PPR virus (PPRV), a Small Ruminant Morbillivirus of the family Paramyxoviridae. The disease is characterised by fever, serous to mucopurulent oculo-nasal discharges, respiratory distress, cough, necrosis and erosions on oral mucosa and diarrhoea. It causes heavy mortality and morbidity among small ruminants, especially goats. The present study aims at molecular detection of N gene of PPRV from the clinical samples collected from goats showing clinical signs of PPR like ocular and nasal discharges, fever, cough and diarrhoea. Nasal swabs, faecal swabs and whole blood in EDTA vials from 15 animals were collected. The N gene of PPRV was detected in two nasal swabs when all the samples were subjected to both real time RT-PCR and conventional RT-PCR. All other samples were negative by both conventional RT-PCR and real time RT-PCR.