Toll Free Helpline (India): 1800 1234 070
Rest of World: +91-9810852116
Free Publication Certificate
Submit Manuscript
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
Vol. 10, Special Issue 12 (2021)
Characterization of indigenous Bacillus thuringiensis isolate T350 toxic to fall armyworm, Spodoptera frugiperda
Author(s):
M Maheesha, V Balasubramani, M Murugan, M Raveendran, G Rajadurai, T Tamilnayagan, E Kokiladevi and N Sathiah
M Maheesha, V Balasubramani, M Murugan, M Raveendran, G Rajadurai, T Tamilnayagan, E Kokiladevi and N Sathiah
Abstract:
Bacillus thuringiensis is widely used in pest management as a biopesticide due to its effectiveness and specificity. Morphological, molecular and insecticidal characteristics of an indigenous Bt isolate T350 toxic to fall armyworm (FAW), Spodoptera frugiperda was studied. The Bt isolate T350 had creamy white, fried egg type colony which is irregular in shape with undulated margin. Bipyramidal shape of crystalline inclusions was found to present in isolate Bt isolate T350. The isolate produced protein bands of ̴ 130 kDa and ̴ 65 kDa size in SDS PAGE analysis and PCR screening confirmed the presence of cry1, cry2 and vip3 genes. LC50 of the isolate was found to be 2.04 µg/ml against the neonate FAW, compared to 1.93 µg/ml for standard strain HD-1.
Bacillus thuringiensis is widely used in pest management as a biopesticide due to its effectiveness and specificity. Morphological, molecular and insecticidal characteristics of an indigenous Bt isolate T350 toxic to fall armyworm (FAW), Spodoptera frugiperda was studied. The Bt isolate T350 had creamy white, fried egg type colony which is irregular in shape with undulated margin. Bipyramidal shape of crystalline inclusions was found to present in isolate Bt isolate T350. The isolate produced protein bands of ̴ 130 kDa and ̴ 65 kDa size in SDS PAGE analysis and PCR screening confirmed the presence of cry1, cry2 and vip3 genes. LC50 of the isolate was found to be 2.04 µg/ml against the neonate FAW, compared to 1.93 µg/ml for standard strain HD-1.
Pages: 1809-1812 | 285 Views 100 Downloads
How to cite this article:
M Maheesha, V Balasubramani, M Murugan, M Raveendran, G Rajadurai, T Tamilnayagan, E Kokiladevi and N Sathiah. Characterization of indigenous Bacillus thuringiensis isolate T350 toxic to fall armyworm, Spodoptera frugiperda. The Pharma Innovation Journal. 2021; 10(12S): 1809-1812.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
