Vol. 10, Special Issue 12 (2021)

Author(s):
Sandhya Suhas Shinde, Sharad Bharat Sanap, Akshay Milind Patil, Nalini Arun Shinde, Abhijit Arun Daspute and Dipak Kachru Sarode

Abstract:
Tobacco (Nicotiana tabacum L.) is a model plant in the field of tissue culture, plant regeneration and plant transgenic. The present study reveals the procedure for the regeneration of tobacco plants by organogenic mode. 3^rd to 4^th node young tobacco leaves were used as explants in this experiment. The callus were formed after leaves cultured onto tobacco regeneration medium supplemented with MS+ 0.1 mg/l NAA+1.0mg/l BAP the percentage of callus produced at this concentration was 55% with a mean size 3.80 cm and mean weight of 5.30 g respectively. The highest numbers of shoots per explants were obtained on the media containing MS+1.0 mg/l BAP +1.0 mg/l NAA which produced shoots per explants.