Vol. 10, Special Issue 12 (2021)

Author(s):
Nidhi Rawat, SD Hirpurkar, Nitin Gade, Jasmeet Singh, C Sannat and Sonali Prusty

Abstract:
Pseudomonas aeruginosa is gram-negative bacilli, known to be one of the causative micro-organisms of mastitis in dairy cattle. A study was conducted to assess the cases of clinical mastitis in dairy cattle associated with the Pseudomonas aeruginosa infection in and around Durg district of Chhattisgarh. A total of 72 milk samples from clinical mastitis cases of cattle were collected after screening by Californian mastitis test. Isolation of Pseudomonas aeruginosa was performed on Pseudomonas agar selective media. Seventeen bacterial isolates showing the blue-green or brown pigmentation were presumptively identified as Pseudomonas aeruginosa which were further subjected to Grams staining and biochemical tests. Molecular confirmation for Pseudomonas aeruginosa was carried out using species specific 16S rRNA gene which revealed 14 confirmed positive isolates. Further, lecithinase activity, gelatinase production, haemolysis and DNase test were also conducted to study the virulence characteristics of confirmed Pseudomonas aeruginosa isolates. This study concludes that Pseudomonas aeruginosa is one of the causative agents commonly reported in clinical mastitis.