Vol. 10, Special Issue 12 (2021)

Author(s):
Avaneesh Kumar Singh, Mahesh Kumar, Dilip Kumar Swain, Vikas Sachan, Jitendra Kumar Agrawal, Anuj Kumar and Atul Saxena

Abstract:
The present study was designed to determine the effect of lycopene as an additive in Hariana bull semen for cryopreservation. Eight ejaculates collected from four Hariana bull (n= 32) were evaluated and those which appears to be apparently normal, having ≥ 3.0 mass motility and ≥ 70% progressive motility were processed further for cryopreservation. Neat semen was extended in egg yolk tris glycerol (EYTG) was splits in to four aliquots and grouped as Group I: control (without lycopene), Group II, Group III and Group IV as treatment group with 0.25 mM, 0.5 mM and 1 mM lycopene respectively. Semen evaluated for acrosomal integrity by Giemsa stain at pre-freeze and post-thaw stage showed that supplementation of 0.25 mM lycopene (Group II) in semen significantly (P<0.01) decreased per cent acrosome reacted spermatozoa at both stages.