Vol. 10, Special Issue 11 (2021)

Author(s):
S Solanki, SK Sharma, K Purohit, M Joshi, A Gaurav and D Devi

Abstract:
The major Objective of this study was focused on using of rapid & efficient methods for detection of Subclinical Mastitis and prevalence of Some Most Important Bacterial Pathogens in Sirohi District of Rajasthan. A total of 200 milk samples from Bovine i.e., Cow and Buffaloes were collected from period of July 2020 to December 2020 to detect and determine pathogens responsible for subclinical mastitis in Bovine. Screening Subclinical Mastitis (SCM) were done using modified California mastitis Test (MCMT) & and Somatic Cell Count (SCC). The prevalence percentage of SCM in California mastitis and Somatic Cell Count in age group of 5 to 8 years Bovines was (74% and 70%) respectively. Identification of the isolates was achieved using Gram’s staining, hemolytic pattern, colony morphology, Catalase, Coagulase test, IMVIC test and confirmation of bacteria species was done by growth on specific agar medium. Bacteriological examination of all milk samples found the presence of (48.00%) isolates where Staphylococcus was predominant species (27.00%) followed by Streptococcus agalactia (10.5%), other streptococci species (4.5%), while environmental pathogen represented E. coli third common pathogen was found in least presence (6.5%). Subclinical mastitis seems to be, as deduced from the high prevalence observed in this study, an important health problem for milking dairy cows and buffaloes in Sirohi. The Identification and biochemical identification is still the important aspect and could be practiced at selected samples from time to time to confirm identification of causative organisms.