Vol. 10, Special Issue 11 (2021)

Author(s):
Ponnusamy P, Sukumar K, Raja A, Saravanan S, Srinivasan P, Balasubramaniam A, Tamilam TV and Thangavelu A

Abstract:
Tracheal samples were collected from desi chickens suspected of infectious laryngotracheitis virus (ILTV) infection. The tracheal samples were triturated and inoculated into embryonated chicken eggs through the chorioallantoic membrane route for isolation of ILTV. After five days of inoculation, chorioallantoic membranes were harvested for histopathological examination. Histopathology of pock lesions on chorioallantoic membrane revealed the presence of syncytia, intranuclear inclusion bodies and infiltration of heterophils and lymphocytes. DNA was extracted from the infected chorioallantoic membrane. Further confirmation, PCR was carried out using the TKOP gene and found positive for infectious laryngotracheitis virus by PCR. This study confirmed the presence of ILT infection in chickens by histopathology and molecular method.