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Vol. 10, Special Issue 11 (2021)

Relationship of in vivo and in vitro post thaw seminal parameters of Bandur Ram semen

Author(s):
Nischitha BM, G Sudha, KS Gunaranjan, GP Kalmath and V Jagadeeshwary
Abstract:
The present study was conducted to evaluate relationship of post thaw laboratory seminal parameters to in vivo fertilization rate in Bandur rams. Five adult Bandur rams were selected for the study with four collections per ram, total 20 collections were used for in vitro evaluation and 100 multiparous ewes were selected for in vivo evaluation. Total of 140 semen straws were used from five rams. From each ram with four ejaculates, 28 semen straws were used and for each collection seven straws of which five straws were used for in vivo evaluation and two straws for in vitro evaluation. The mean per cent obtained was post thaw motility of 35.40 ± 2.25, 44.53 ± 2.71 of viability, 33.80 ± 3.01 of plasma membrane integrity, 96.01 ± 0.25 % of acrosome integrity and 8.55 ± 0.79 per cent of total morphological defects (head defect 2.50 ± 0.44 %, mid piece defect 2.33 ± 0.28 % and tail defect was 3.33 ± 0.28 %). The mean conception rate obtained at field level was 43.00 ± 3.64 %. No significant relation was established in between laboratory parameters and in vivo conception rate. From the field study it is concluded that the results of artificial insemination vary between the flocks and largely depends on the nutritional status of the ewes and conception rate was affected by detection of estrus, depth of penetration of the cervix and skill of the inseminator.
Highlights
. In vitro evaluation of post thaw semen quality in Bandur rams.
. Relationship of in vitro evaluation to in vivo fertilization rate in Bandur rams.
. No significant relation was found in between in vitro and in vivo fertilization.
Pages: 920-923  |  178 Views  24 Downloads
How to cite this article:
Nischitha BM, G Sudha, KS Gunaranjan, GP Kalmath and V Jagadeeshwary. Relationship of in vivo and in vitro post thaw seminal parameters of Bandur Ram semen. The Pharma Innovation Journal. 2021; 10(11S): 920-923.
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