PCR assay of Toxocara canis from soil isolates in and around Hyderabad, Telangana state, India
K Anusha, M Udaya Kumar, GS Sreenivasa Murthy and P Kalyani
The soil borne parasites extract a huge toll on the health and well-being of global population and are a major source of morbidity and mortality affecting millions of people as well as livestock. Among soil borne parasitic zoonoses, Toxocarosis is the major infection causing Visceral larva migrans. The presence of infective larva forms in the soil is an indicator of potential risk to both human and animals.About 230 soil samples were collected from four different locations in and around Hyderabad region of Telangana state, India. All the samples were processed for isolation and confirmed with Polymerase chain reaction (PCR). Of all the samples screened, Toxocara canis isolated from 39 soil samples of 380bp product size amplicons on agarose gel electrophoresis which is 16.95% prevalence and could detect as low as 0.781 ng of gDNA of Toxocara canis which was equivalent to even less than an egg DNA. By considering both the sensitivity and specificity it is concluded that the PCR assay standardized in the present study can be used as molecular diagnostic tool for specific detection of Toxocara canis egg DNA in soil samples, where visceral larva migrans is prevailing among human children.
How to cite this article:
K Anusha, M Udaya Kumar, GS Sreenivasa Murthy and P Kalyani. PCR assay of Toxocara canis from soil isolates in and around Hyderabad, Telangana state, India. The Pharma Innovation Journal. 2021; 10(11S): 887-891.