Vol. 10, Special Issue 11 (2021)

Author(s):
Shruthi K, DH Patel, Rajkumar BK, RK Patel and KG Modha

Abstract:
Cotton plant which is also known as white gold is cultivated all around the world. It is the main source of raw material for the textile industry and also contributes to edible oil. In this study to analyze the genetic diversity of 30 cotton genotypes, Randomised Amplified Polymorphic DNA (RAPD) was used. A total of 21 RAPD primers were used for screening 30 germplasm lines out of which 152 fragments were amplified with 21 random primers, out of which 76.44% were polymorphic. Genetic similarity matrix based on Jaccard similarity coefficient of cotton genotypes ranged from 0.62-1.00. These coefficients were used to construct a dendrogram using the Unweighted Pair Group of Arithmetic Means (UPGMA). All 30 cotton genotypes were grouped into two clusters, which were further subdivided into sub-clusters. The highest similarity has been observed between GISV208 and GISV 246. The present study indicated a great diversity among these 30 genotypes. The RAPD technology proved to be a potentially simple, rapid, reliable, and effective method for detecting polymorphism to assess genetic diversity among genotypes and very helpful in selecting diverse parents for hybridization.