Toll Free Helpline (India): 1800 1234 070

Rest of World: +91-9810852116

Free Publication Certificate

Vol. 10, Special Issue 10 (2021)

Cell-mediated immune response against F-1 and LaSota strains of new castle disease virus in chicken

Author(s):
Rajesh Singathia, Ravindra Sharma and Satish Kumar Batra
Abstract:
F-1 and LaSota strains of New castle disease were propagated in the laboratory using embryonated hen eggs via the allantoic cavity route. One group of thirty chicks were infected with F-1 strain and the other with LaSota strain with virus via oral and ocular route. Sequential lymphocyte from blood samples at days 0, 3,7,14, 21, and 28 post infection were collected and cell mediated immune response against F-1 and LaSota strains of New castle disease virus (NDV) at different days post inoculation was analyzed by performing lymphocyte proliferation assays and by fluorescence-activated cell sorting analysis of phenotypic markers of T lymphocytes. In both the group of chicks immunized with F-1 and LaSota strains of NDV, lymphocyte proliferation responses at different days post infection were significantly higher (P<0.05) compared to that of the control group on in vitro stimulation with antigen. On in vitro stimulation with Concanavalin A the mean stimulation index values in the group of birds immunized with F-1 or with LaSota strain of NDV were found to be significantly higher than that of control at most of the days. A statistically lower (P<0.05) proportion of CD4+ T lymphocyte was observed on days 3 & 7 post infection in group of chickens inoculated with either F-1 or LaSota strain of NDV as compared to that of control birds. A lower proportion of CD8+ T cells were observed on days 7 & 14 post infection in birds inoculated with either F-1 or with LaSota strain of NDV. The Cellular immune response is generated in the form of first suppression (day 3) followed by an increase in CD4+ T lymphocytes and CD8+ T lymphocytes.
Pages: 1370-1377  |  376 Views  131 Downloads
How to cite this article:
Rajesh Singathia, Ravindra Sharma and Satish Kumar Batra. Cell-mediated immune response against F-1 and LaSota strains of new castle disease virus in chicken. The Pharma Innovation Journal. 2021; 10(10S): 1370-1377.

Call for book chapter