Vol. 10, Special Issue 10 (2021)

Author(s):
Lhingneivah Doungel, Indu VR, Lucy KM, Vasudevan VN, Sunilkumar NS, Justin Davis and Gleeja VL

Abstract:
The present study was undertaken to assess the physico-chemical properties of decellularised tunica vaginalis of buffalo. For decellularisation, the fresh parietal layer of tunica vaginalis (PTV) procured from testes of ten adult healthy Murrah buffalo between two to five years of age, slaughtered at Meat Technology Unit, College of Veterinary and Animal Sciences, Mannuthy. The samples were cut into 2cm × 2cm square pieces, washed with deionised water and stored in high-density polyethylene pouches at -20 °C. Defatted by immersing overnight in mixture of chloroform and methanol (2:1 v/v) at room temperature. Decellulularisation by Trypsin-EDTA protocol utilising Triton-X-100 as detergent. The physico-chemical properties were analysed and compared to raw parietal layer of tunica vaginalis. The decellularised PTV samples showed acceptable physico-chemical characteristics. Mean thickness, tensile strength and collagen concentration of decellularised samples were significantly reduced while the collagen solubility per cent and percentage weight loss after collagenase degradation were significantly higher in decellularised PTV than raw PTV samples.