Vol. 9, Special Issue 7 (2020)

Author(s):
K Nishitha, SB Rahmaan, SS Shiji, IS Sajitha and V Babitha

Abstract:
Angiopoietin-1 (ANPT-1) is an angiogenic growth factor, and a ligand for the receptor-“Ties” (a family of receptor tyrosine kinases). Present study aimed to determine the differential protein expression of Angiopoietin-1 in ovarian follicles of cross bred Malabari goats by immuno-histochemical method. Protein expression of Angiopoietin-1 gene could be detected in the ovarian follicular cells viz., granulosa cell, theca interna cells, endothelial cells of blood vessels, follicular fluid and germinal epithelium. Also it was observed that percentage of positivity (number of positive cell/ total number of cells in the follicle) for Angiopoietin-1 was higher in initial stage of follicular development when compared with later stages of follicular development, hence it was concluded that the protein expression of Angiopoietin-1 decreases with increase in follicular size. The staining intensity and the number of positive staining cells were more in the granulosa cell layer than in the theca interna cells of antral follicle. Overall scoring of staining intensity was also more in granulosa cell layer compared to the theca interna cells.