Vol. 9, Special Issue 4 (2020)

Author(s):
Sameena, A Vijaya Kumar, Sujatha Sing, N Krishnaiah and A Poojasree

Abstract:
The aim of this study was to estimate the seroprevalence and molecular detection of Coxiella burnetii in sheep and goat milk of Telangana state. A total of 36 pooled milk samples (17 sheep and 19 goat) were collected from different areas of Telangana state. The milk samples were tested by enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction with specific primers trans and com 1 gene. In the present study, out of 36 pooled milk samples 12 (33.33%) and 16 (44.44%) were positive by ELISA and PCR respectively. Out of 17 sheep and 19 goat milk samples 5 (29.41%), 7 (36.48%) were tested antibodies positive against Coxiella burnetii using commercial ELISA kit and 7 (41.18%), 9 (47.37%) by PCR respectively.