Vol. 12, Special Issue 7 (2023)

Author(s):
Nishant Ranjan, Pallavit Lata, Shristi Tanaya, Shivam Shekhar, Arpit Raturi, Rishav Kumar, Dheetan Parthsarthi, Vijeta Jha, Sushant Kumar and Viabhav Kumar Upadhayaya

Abstract:
Genome editing is a technique that has opened the door for a new concept in which we can manipulate the genome sequence for different purposes. Generation of specific pre-planned changes for genome manipulation includes double-stranded breaks. The double-stranded breaks use the intrinsic repair mechanism of the cell. Four major methods for inducing site-specific double-stranded breaks which are CRISPR, TALENs, ZFN, and Mega nuclease. Out of these CRISPR is the most recent and easy-to-use technique for genome editing. It has provided powerful tools for the precise modification of genetic material. Genome editing has a wide range of purposes in different sectors including healthcare, therapeutics, agriculture, etc.