Studies on dose optimization, genetic variability and mutagenic effects through gamma irradiation in gladiolus (Gladiolus grandiflorus L.)
Author(s):
Amitesh Gendle, Pooja Gupta, Rinu, Tarun Kumar, and Khusboo Sharma
Abstract:
In Randomized Block Design with Factorial Principle, the experiment was planned with three replications. Four gladiolus cultivars (Pusa Suhagan, Arka Pratham, Arka Amar, Punjab Dawn) and two gamma ray doses and control doses were used to administer the procedure (25, 35). The radio-biological effects of VM1 generations on sprouting, survival and other morphological attributes have been investigated. Plants treated with higher doses (35 Gy) showed deleterious effect of ionising radiations although at lowest dose (25 Gy) plants were not affected much. Minimum number of days taken to sprouting (10.00 days), maximum plant height observed in the 25 Gy treatment and at par to the untreated (control) gamma rays dose at 35 Gy and Flower diameter (8.61 cm) and length of spike (81.70 cm) was slightly increased at 25 Gy and length of rachis (40.53 cm) at 25 Gy treatment as compare to untreated (control) plants. Minimum days to sprouting, Maximum spike length, rachis length and flower diameter was recorded in the interaction of variety Pusa Suhagan and Arka Pratham with gamma ray dose at 25 Gy, respectively. Homeotic mutants were isolated from all the varieties at 25 and 35 Gy.
How to cite this article:
Amitesh Gendle, Pooja Gupta, Rinu, Tarun Kumar,, Khusboo Sharma. Studies on dose optimization, genetic variability and mutagenic effects through gamma irradiation in gladiolus (Gladiolus grandiflorus L.). Pharma Innovation 2024;13(3):18-21.