Vol. 12, Issue 9 (2023)

Author(s):
MM Rifana Ajam, Sobita Devi, Bireswar Sinha, L Nongdrenkhomba Singh, N Gopimohan Singh, Khumukcham Ibohal Singh, Nancy Khwairakpam, M Victoria Chanu, O Yaiphabee, Neha Soibam, Surbani Laishram, N Nita Devi, N Niranjit Singh, H Brajesh and GP Sharma

Abstract:
Trichoderma spp. is the bio-control agents intensively used in the field of agriculture for diseases management. Trichoderma species are now well recognised as effective biocontrol agents for a number of industrial phytopathogens. For the current experiment, four native isolates of Trichoderma asperellum were collected from Plant Pathology Department, College of Agriculture, CAU, Imphal. In this research, pH, temperature and sugar concentrations at different levels were considered to study the isolates of mycelial growth variation of T. asperellum. Optimum pH, temperature and sugar concentrations were studied on potato dextrose agar media were prepared at different pH 4, pH 5, pH 6, pH 7 and pH 8, temperature 26 ˚C¸ temperature 28 ˚C¸ temperature 30 ˚C¸ temperature 32 ˚C and temperature 34 ˚C) and sugar concentrations (10 g/L, 15 g/L, 20 g/L, 25 g/L and 30 g/L). For optimization, at pH 5 the maximum radial growth was obtained whereas fresh weight and dry weight was obtained at pH 5 and pH 6 respectively. The maximum radial growth was observed at temperature 28˚C and 30˚C and fresh weight and dry weight was observed at temperature 34 ˚C. At 20 g/L of sugar concentration, the maximum radial growth as well as fresh weight and dry weight of the mycelium were found to be suitable. The results of this investigation demonstrated that the growth of T. asperellum isolates is greatly influenced by the pH, temperature and sugar concentration. The results of the current study showed that the growth of the isolates of T. asperellum was significantly influenced by environmental factors.