Vol. 12, Issue 8 (2023)

Author(s):
Niresh Kumar Sivararamakrishnan, Satya Vijayalakshmi Kothandaraman, Renukadevi Perumal, Malathi Varagur Ganesan, Saranya Nallusamy and Thiyagu K

Abstract:
Mungbean yellow mosaic virus (MYMV) is one of the most destructive begomoviruses, which infect legumes and cause significant yield losses. The whitefly transmission of MYMV in susceptible variety (CO₅) resulted in symptom expression in seven out of ten plants, whereas in resistant variety (Mash 114), symptom expression was absolutely absent in all the ten plants. Further, in MYMV detection through PCR using Rep region (DNA-A) and movement protein gene (DNA-B) specific primers, all the ten plants were PCR positive for DNA-A and eight for DNA-B in the case of CO₅, in which seven were symptomatic and three were asymptomatic. In the case of resistant variety Mash 114, eight out of 10 plants were PCR positive for both DNA-A and DNA-B, but were asymptomatic. The different seed parts viz., seed coat, cotyledon and embryonic axis from the whitefly transmitted, yellow mosaic disease infected plants exhibited the presence MYMV in all the seed parts in the case of CO₅, whereas in Mash 114, the virus was present in seed coat and cotyledon, but absent in embryonic axis. The study confirmed that the MYMV presence in embryonic axis of the susceptible variety CO₅ promotes the probability of seed transmission.