Vol. 12, Issue 5 (2023)

Author(s):
Nairita Vaidya, Iswar Chandra Mohanty and Karishma Behera

Abstract:
Iron toxicity is one of the main abiotic stresses affecting rice, usually seen in flooded rice environment. Locating out a closely linked marker that is linked with the trait is necessary so that it can be employed in Marker assisted selection for development of HYVs tolerant to iron toxicity. A number of markers linked to the trait have been reported, but these markers are needed to be validated for their further applications. In the present research, 150 germplasms which include land races and HYVs were grown in hotspots for phenotyping to assess tolerance to iron toxicity. Then, 30 germplasms were genotyped using 7 SSR markers earlier reported to be linked to Fe toxicity trait in rice for validation. After running of PCR, 3 SSR Primers were found to show bands of reported amplicon size in tolerant and moderately tolerant species and were hence found to be validated and can be further utilized for MAS.