Vol. 12, Issue 5 (2023)

Author(s):
Ch. Aruna Kumari and Sameena Begum

Abstract:
The consequences of climate change, new fungal infections, the desire for fewer chemicals on the field and high-quality agricultural products are the major challenges for sustainable agriculture. Plant breeders respond to all these challenges with new varieties, using the most suitable breeding methods. In the last decade there has been a dramatic expansion of new genetic engineering and biological tools: genomics data; metabolic engineering; high-throughput analytical tools, including whole organism gene expression analysis and metabolomics and powerful genome-editing technologies. These new breeding methods complement farmer’s tool boxes and offer additional opportunities to enhance plant breeding. Among these, the bacterial clustered, regularly interspaced, short palindromic repeat (CRISPR) associated protein 9 (Cas9) or CRISPR-Cas9 has emerged as the easiest, most economic and efficient technology to undertake genome editing. This technique allows precise site-specific gene modification or integration. This review summarizes the application of CRISPR/Cas9 system in rice.